Part:BBa_K4160006
STAT promoter + CMVmin
This part encodes for the STAT promoter, which can bind STAT proteins. Upon binding of STAT proteins, STAT-induced genes will be expressed.1 In this part, the STAT promoter is accompanied by a cytomegalovirus (CMV) sequence. This sequence is a commonly used enhancer element that allows for the high-level production of recombinant proteins in mammalian cells.2
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 53
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 53
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 53
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 53
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The STAT promoter is a DNA sequence that regulates the transcription of downstream STAT-induced genes.1 The STAT promoter induces gene transcription upon binding of the transcription factor STAT3 (BBa_K4160005). STAT proteins bind to DNA recognition motifs, named gamma-activated sites (GAS), that are located in the STAT promoter sequence. This binding initiates the transcription of downstream genes.3
GEMS system
TU-Eindhoven 2022 used this part as a transcription factor for the Generalized Extracellular Molecule Sensor (GEMS) system. Upon dimerization of the GEMS receptor, IL-6RB (BBa_K4160002) domains are activated and induce downstream signaling of the JAK/STAT pathway, which results in STAT-induced gene expression.
Characterization
Characterization of expression of STAT-induced proteins in the GEMS system can be found on the BBa_K4160008 page.
References
None |